Xiao Lu Yin
Soochow University, P R China
Title: Gynura divaricata rich in dicaffeoylquinic acid and chlorogenic acid restrains islet cell apoptosis and improves pancreatic function in type 2 diabetic mice
Biography
Biography: Xiao Lu Yin
Abstract
The aim of this study is to investigate the potential protective effect of Gynura divaricata (GD) in diabetic mice. Gynura divaricata (GD) is a kind of natural products for edible and medicine. The analytical results showed that the methanol extract of GD riches in 10.3% 3, 5- or 6.8% 4, 5-dicaffeoylquinic acid and 5.6% chlorogenic acid by high-performance liquid chromatography with diode array detection (HPLC–DAD). After STZ-induced 2 type diabetic mice (male ICR) were orally administered with 1%, 5% and 10% GD diet for 4 weeks, a series of assays was performed. The hypoglycaemic rate of fasting blood glucose (FBG) reached 41.4% in 10% GD group and the fasting serum insulin in the highest dose GD group has decreased as low as normal group. GD treatment significantly reduced the glycosylated serum protein (GSP) level in diabetic mice. The activities of total superoxide dismutase (SOD) and glutathione peroxidase were markedly increased; while the malonaldehyde (MDA) level was decreased in pancreas tissue by treatment of GD. HE-staining showed that the pathological damage in islets with pancreatic b-cells was ameliorated by GD treatment. An immunohistochemical assay showed that GD promoted marked b-cell regeneration. GD treatment also caused notable increase in GLUT2, GK, MafA, PDX-1, and Bcl-2 as well as reduction in Bax and caspase-3 expression as shown by Western Blot analysis. In conclusion, GD exerts the pronounced hypoglycaemic effect by restraining islet cell apoptosis and improving pancreatic function. Therefore, GD might be a promising food or medicine for the treatment of diabetes